The Transcriptional Landscape of the Early Embryonic Developmental Biology in Hulunbuir Short-Tailed Sheep

Background: Since both fetal and postnatal development is determined by early embryonic the molecular mechanism organogenesis process of mouse embryos have been thoroughly studied. However, specific in Mongolian sheep remains poorly understood. Methods: The 16-day 25-day embryo samples Inner Mongolia Hulunbuir short-tailed were evaluated transcriptome sequencing technique genes with significant differences screened gene function was enriched ontology (GO) database Kyoto Encyclopedia Gene Genome (KEGG) database. Result: A total 4348 differentially expressed (DEGs) analyzed especially DEGs included such as COL6A2, COL4A4, LAMA4, ITGA8, FN1 involved ECM-receptor interaction, focal adhesion, PI3K-Akt signaling protein digestion absorption pathways; YAP1, FZD4, TGFB1, WNT2, BMP5, BMP2, WNT11 Hippo pathway. In pathway analysis, those pathways mainly related to cell interaction highlighted at E16 stage. They are critical for transcriptional control development, during organ building. that make organs body structures more complex detailed brain, liver immune system E25 Therefore, this study provides important supplementary exploratory reference data further research on sheep.